Plasmid Preparation:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Sequencing:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Binding Assay:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Methylation:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Expressing:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Activity Assay:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: Construction of STARR-seq_Alb plasmid. .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARRseq_SCP1 was modi ed to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDItransfected livers [59]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
Modification:Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [ ]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [ ]. ..
Article Title: HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Article Snippet: .. Plasmids hSTARR-seq_SCP1 vector (Addgene plasmid # 99292, RRID:Addgene_99292; listed as pTYC6 in Table S1E) and hSTARR-seq_ORI vector (Addgene plasmid # 99296, RRID:Addgene_99296; pTYC7 in Table S1E) were gifts from Alexander Stark [25]. hSTARR-seq_SCP1 was modified to generate plasmid pTYC10 by removing the SCP1 promoter sequence by BanII digestion and replacing it with a minimal mouse Alb promoter sequence, comprised of 318 nt obtained from pLIVE plasmid (cat. #MIR 5420, Mirus Inc, Madison, WI), where the Alb promoter HNF1 TF binding site was changed from GATC to AATC to prevent bacterial methylation and to enable expression of the full intrinsic activity of the Alb promoter in HDI-transfected livers [61]. ..
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